TestoFuel: Scientific Review on Ingredients, Dosage, and Side Effects
TestoFuel: An Evidence-Based Review

TestoFuel: A Scientific Review on Ingredients, Dosage, and Side-Effects

Review of: TestoFuel Natural Testosterone Booster


Some proven ingredients


Mostly positive feedback


May cause digestive issues


Higher price point


  • Powerful micronutrient support
  • Improved mood and mental clarity 
  • Libido support
  • No proprietary blends


  • Unproven test boosting ingredients​​​​
  • exclamation-triangle
    Relatively expensive
  • exclamation-triangle
    Lacks hypertrophy support
  • exclamation-triangle
    Testimonials seem fishy

Summary: TestoFuel packs in some powerful vitamins and minerals that have been proven to increase testosterone. As per its herbal ingredients, (D-aspartic acid, fenugreek, and ginseng), they have mixed evidence surrounding their effectiveness.

Verdict: May increase natural testosterone production.

Disclaimer: I do not have personal experience with TestoFuel. My review is based on an analysis of the research behind each one of its ingredients.

My Rankings of the Best Test Boosters on the Market


TestoFuel's proven ingredients are in the vitamin and mineral formula. The primary ingredient, D-Aspartic Acid, has been shown to have some positive influence on testosterone, but further research is needed to confirm this effect. 

My recommendation is always to use supplements for what they're made:

To supplement your diet and lifestyle.

If you're reading this right now with the intention to use TestoFuel as the sole means to optimize your testosterone levels, then definitely DO NOT buy it. Only make this investment if you are serious about taking control of your health, and willing to align your nutrition, exercise, sleep, and other lifestyle factors with that goal.

So to answer the question:

Yes, TestoFuel does work to naturally increase testosterone levels.


The extent of this increase will be directly proportional to the efforts you make in fixing your diet and lifestyle. 


My supplement reviews are based on three criteria: Ingredients, Testimonials, and Price. 

The most important part of this review is the ingredients analysis. Once you understand the implications of the research behind each of the ingredients, you'll have much better insight towards how they actually go on to affect your body.

TestoFuel Ingredients: 4/5

TestoFuel Ingredients

Vitamin D3

Vitamin D supports 1000s of bodily functions and is essential for human survival. The sun provides the major source of vitamin D, but it is also present in foods such as fish, eggs, and dairy. 

Supplementing with vitamin D is associated with a host of health benefits including (but not limited to) stronger bones, a stimulated immune system, and increased cognitive function. 

As per it's affects on testosterone, here's what the research says:

  1. Men with sufficient vitamin D levels have significantly higher testosterone levels compared to men who are deficient in it (1). 
  2. Men supplementing with 3332 IU of vitamin D for one year were found to have, on average, 25% higher testosterone levels compared to placebo (2). 

Depending on the extent of your current vitamin D deficiency, topping up your levels can have a dramatic impact on your testosterone levels. Even having sub-optimal levels of it can lead to sub-optimal T. 

With 5000 IU of it per serving, TestoFuel has your vitamin D requirements covered.  

According to the British Medical Journal, vitamin D deficiency is the most common nutritional deficiency worldwide, in both children and adults (3). So don't take this lightly.


Vitamin K2

Vitamin K2 is associated with a host of health benefits, the most prominent of which include preventing heart disease (4) and increased bone mineral density (5).

So how does it impact testosterone?

There have been no human trials in this area, but some rodent studies provide interesting results:

  1. Rats given 75mg of vitamin k2 per kg of bodyweight experienced nearly a doubling of their testosterone levels (6).
  2. In another study, vitamin K deficiency in rats significantly reduced their testosterone levels (7). 

Vitamin K2 can provide tremendous benefits and should be included as a part of any healthy diet. It has also been shown to be associated with significantly higher testosterone levels in rats. Whether this effect carries on to affect us in the same way is hard to say.


Vitamin B6

Vitamin B6, aka pyridoxine, has a part to play in many bodily functions but is primarily involved in synthesizing protein (8). It is naturally present in fruits and vegetables, but can also be taken as a supplement. 

With regards to its impact on testosterone, there have been no human studies but rodent studies show a positive correlation: 

  1. Rats deficient in vitamin B6 had significantly lower testosterone levels (9).
  2. High levels of vitamin B6 reduced estrogen-induced gene expression by 30%. Vitamin B6 deficiency, in-turn, increased estrogen-induced gene expression by 85% (10). 

These results are definitely interesting, but without any human trials, it's hard to say whether this effect carries on to us as well. 

In one human study, subjects were put on a stationary bike whereby they pushed to 80% of their heart rate. Subjects given 600mg of pyridoxine noted greater increases in growth hormone and lower prolactin levels compared to placebo (11). 

So vitamin B6 may impact testosterone indirectly:

Both higher GH and lower prolactin may translate to higher T.

These studies are definitely not conclusive and more trials are needed to confirm these results. Nonetheless, vitamin B6 does aid overall health and the addition of it in TestoFuel can't hurt. 



​Magnesium is a key mineral in the human body that controls a variety of functions: heart health, bone health, blood pressure, and insulin sensitivity being just a few of the more prominent ones. In developed countries, magnesium is the second most common micronutrient deficiency after vitamin D. This is because grains, which make up a large portion of the western diet, are devoid of magnesium. Simply including more nuts and leafy greens in your diet can help top up your levels. 

If your current magnesium levels are deficient, then topping them up can have a serious positive impact on testosterone. That being said, if your magnesium levels are adequate, then topping them up will not likely impact your testosterone. 

Here's what the research says:

  1. After evaluating the health data for 399 older men, researchers found that magnesium levels were strongly and independently associated with testosterone levels (12). 
  2. Athletes and sedentary subjects supplementing with magnesium, over the course of 4 weeks, were found to have higher testosterone levels (13). 

TestoFuel provides 50% of the daily recommended value for magnesium. 

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Zinc is another micronutrient that is essential for human survival. Any list of testosterone boosting foods that you read online will most likely have foods on there due to their high zinc content. Animal products, eggs, and beans all good sources of zinc.

Like the other micronutrients we've talked about so far, zinc supplementation will impact your testosterone levels only to the extent that you are deficient in it. At high doses, though, zinc can also act as an aromatase inhibitor, i.e. it reduces estrogen levels. 

Let's take a quick look at research:

  1. Wrestlers supplementing with zinc (3mg/kg/day) for 4 weeks were found to have significantly higher testosterone levels (14).
  2. After exercising on a stationary bike, subjects experienced a decrease in testosterone levels. Supplementing with zinc, however, prevented this decrease (15). 

Zinc is lost through sweat. So if you're exercising daily or you live in a hot environment, zinc supplementation can be very important for you. But then again, keep in mind that if you already have balanced zinc levels, supplementing with it won't do much for your T. 

As for zinc's status as an aromatase inhibitor:

  1. Rats with a zinc deficiency showed a 57% increased expression of estrogen receptors (16).  

TestoFuel provides 67% of the daily recommend​ed amount for zinc. 


D-Aspartic Acid

​D-AA is amongst the most common ingredients found in many testosterone boosters. There's been a bunch of research studying its interaction with testosterone, but results are inconclusive. 

I'll run you through some of the major studies so that you can decide for yourself whether it works or not:​

  1. 23 men were given a daily dose of 3g of D-aspartate for 12 days, after which they experienced 42% higher testosterone levels (17). 
  2. Resistance trained men supplementing with 3g of D-AA for 28 days experienced no differences in T-levels relative to placebo (18).  
  3. Infertile men supplementing with 2.66g of D-AA for 90 days experienced between 30-60% higher testosterone levels (19). 
  4. 24 subjects were given either 3g of D-AA, 6g of D-AA, or placebo for 14 days, during which they also lifted weights. Researchers found that, at the end of the study, 6g of D-AA supplementation actually lowered testosterone levels relative to placebo. The subjects on the 3g dose didn't experience any significant differences (20). 

D-AA is the primary ingredient in TestoFuel, but the results regarding its interactions with testosterone are mixed. At this point, further research is needed to solidify D-AA's status as a potent test booster for healthy males. That being said, if your current testosterone levels fall far below the normal range, then D-AA may help in getting them back closer to where they should be. 


Asian Red Panax Ginseng 

​Panax ginseng is the most studied form of ginseng. It seems to be effective at boosting mood, immunity, and brain function. As for its effects on testosterone, let's dive in to the research:

  1. 60 men with mild or moderate ED were given either 1g of panax ginseng or placebo. At the end of the study, the men receiving ginseng had improved markers of penile performance (21). The effect on testosterone levels was not statistically significant. 
  2. Men with poor sperm mobility experienced significantly higher testosterone levels after supplementing with red panax ginseng (22). 
  3. In yet another study, supplementing with red panax ginseng was found to be an effective treatment for ED (23). 

The bottom line is that Asian Red Panax Ginseng is an effective nitric oxide booster. It dilates the blood vessels and improves blood flow throughout the body. As per its direct effects on testosterone, the studies are not conclusive. Even the one I mentioned above was performed on men with low sperm mobility. That being said, my conclusion is that ARPG is an effective nitric oxide booster and potential testosterone booster. As per its host of other benefits, it seems to make a good addition to the TestoFuel formula. 



Fenugreek is a popular herb from India that has traditionally been used to boost libido. More recently, it has found its way into the list of ingredients for many popular testosterone boosters. ​Let's take a look at what the research says:

  1. 30 resistance trained men were given either 500mg of fenugreek or placebo. They trained 4 days per week. After 8 weeks, the men taking fenugreek had significantly higher testosterone levels and lower body fat (24). 
  2. 60 healthy males were given 600mg of Testofen (fenugreek) for 6 weeks. At the end of the study, although testosterone levels were not significantly affected, an increase in physiological aspects of libido were noted (25). 
  3. 45 resistance trained men were given 500mg of fenugreek. After 8 weeks of supplementation, no significant changes in testosterone were noted, although 9.42% decrease in DHT was noted (26). 

Once again, the studies show mixed results. Although the effect on testosterone will be negligible, fenugreek may help with increasing your sex drive. As for its effect on DHT (an anabolic hormone with similar effects as testosterone) don't worry too much, because the experiment used a dose of 500mg. TestoFuel contains only 100mg. 


Oyster Extract​

​Oysters are perhaps the most popular testosterone-boosting food, and its because of their high zinc content. As mentioned, zinc is a key mineral that has been studied for its interactions with anabolic hormones. There is a direct correlation between zinc levels and testosterone levels. 

That being said, if you already have balanced zinc levels, then topping up your zinc further will not affect your testosterone. The extent to which zinc will impact your testosterone levels is dependent on the extent of your current deficiency. ​

Testimonials: 3.5/5

​There are some pretty insane before/after pictures on the TestoFuel website, but in my opinion these results seem exaggerated. Although supplementing with TestoFuel may have been a factor in the transformations, it was definitely not the only factor. Such dramatic physique changes can only occur by making significant changes in diet, training, and lifestyle. 

Don't buy TestoFuel with the expectation that it will add pounds of muscle to your body. If you want to build a lean and muscular physique, diet and exercise should be made priority. Only use TestoFuel to provide you with an edge once those areas are handled. 

Price: 3/5

1 box of TestoFuel retails for $65 (about $2 per serving). 

The other option is to buy 2 boxes for $130, and you get free shipping in the UK and US.

The third option is to get 4 boxes of TestoFuel for $195. That comes out to about $1.60 per serving. Plus, they also offer free world-wide shipping and two free eBooks with this option. 

TestoFuel is not available on Amazon, GNC, or any place else. You'll have to check out their website if you want to order it. ​

TestoFuel Side-Effects

The ingredients in TestoFuel are safe. The side-effects listed below are associated with taking too much of any particular ingredient.

Remember to always read the label and follow instructions. Too much of anything is a bad thing.

You are unlikely to experience these side-effects if you stay within the recommended dose. 

Possible Side-Effects of D-Aspartic Acid​

  • Hair loss
  • Acne
  • Bloating
  • Gynecomastia


Possible Side-Effects of Asian Red Panax Ginseng​

  • Difficulty sleeping
  • Headache
  • Agitation
  • Upset stomach
  • Dizziness


Possible Side-Effects of Fenugreek

  • Diarrhea 
  • Upset stomach
  • Bloating
  • Gas
  • "Maple syrup" odor in urine
  • Nasal congestion
  • Coughing
  • Facial Swelling
  • Wheezing
  • Allergic reactions
  • Lower blood sugar in diabetics


Possible Side-Effects of Oyster Extract

  • Nausea
  • Vomiting
  • Diarrhea
  • Metallic taste
  • Kidney and stomach damage



How does TestoFuel stack up against some other testosterone booster supplements on the market? 

Well, what it ultimately comes down to is ingredients.

Below, I've listed out quick comparative summaries on TestoFuel versus Testogen, Status, P6, and Test Freak.

TestoFuel vs TestoGen

The primary ingredients in Testogen, like in TesoFuel, are D-AA, Fenugreek, and Ginseng. The additional ingredient in Testogen is Tribulus.

Tribulus is a popular ingredient in many testosterone boosters, and while it appears to boost libido and fertility, it has been proven to do next to nothing for testosterone levels (27). 

Testogen also packs in some extra vitamins and minerals like selenium, B vitamins, and riboflavin. 

In terms of ingredients, both of these supplements will most likely affect your body in the same way.  

TestoFuel vs Status

Status is a testosterone supplement manufactured by Blue Star Nutraceuticals.

The primary ingredients in Status are Ashwagandha, Tongkat Ali, and DIM. Each of these has been shown to have some positive interaction with testosterone.

Tongkat Ali needs more research to support its effectiveness, but DIM is a proven aromatase inhibitor (28) and Ashwagandha has been studied for its positive effect on T (29).

TestoFuel has an ingredient profile more suited towards helping you optimize your T.

TestoFuel vs P6

P6 is a testosterone support supplement manufactured by Cellucor. 

Cellucor has released a bunch of other bodybuilding supplements, and this is its flagship testosterone booster. ​

As far as the ingredients go, they are comprised in a proprietary blend, and we have no idea of the doses. 

From the looks of it, ashwagandha is the primary ingredient along with a bunch of other herbal extracts. ​

In my opinion, P6 may be good for overall health, but as far as its interaction with testosterone goes, it probably won't affect it too much. ​

TestoFuel versus P6

TestoFuel vs Test Freak

Test Freak is a supplement manufactured by the company Pharma Freak. It contains a blend of zinc, magnesium, and B vitamins along with three other proprietary blends. 

The first blend is comprised of fenugreek and tribulus. As you know fenugreek has mixed results about its interaction with testosterone. Tribulus, on the other hand, is proven to not be effective.

The second blend is ​meant to increase DHT levels, but I was unable to find any research supporting the ingredients ability to do so. 

The third blend is meant to be an estrogen blocker blend. Although there are some proven ingredients, I don't think ​it would do much. 

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